LAB Basic Course in Laboratory Skills of EBSOT
The European Basic Science in Organ Transplantation (EBSOT) Committee promotes basic technological and scientific research applied to organ and cellular transplantation within the European Society for Organ Transplantation (ESOT). EBSOT aims to contribute to the communication, education and support of basic science activities in transplantation. Among its activities, EBSOT organizes “The Basic Course in Laboratory Skills”. This course aims to expand the knowledge of laboratory skills within ESOT and to create an environment that stimulates collaboration between scientists.

 

Objectives
The course aims to teach the laboratory techniques applied to investigate various aspects of immune responses in experimental and clinical transplantation research. The Nantes course will address several techniques used in the isolation, characterization and analysis of dendritic cells (DCs) both in mice and humans. DCs play a central role in the mechanisms of allograft rejection and tolerance. The INSERM U643 Laboratory has a large experience in the study of human and rodent dendritic cells in transplantation models and in their basic biology.

Target group
PhD students, young scientists and physicians directly involved in laboratory research involving or planning to use DCs are encouraged to enroll. Previous basic knowledge on flow cytometry and cell culture techniques are necessary.
The Basic Course in Laboratory Skills of EBSOT
Nantes July 1-2, 2010
 
 
Thursday 1
 
09h30 - 10h30    INTRODUCTION                                                                                    
                         Régis Josien, Nantes, France

10h30 - 13h30    Group 1 - Generation of human DC from monocytes.                                 
                         Séverine Remy, Nantes, France; Gaelle Tilly, Nantes, France
  Group 2 - Purification of human pDC and cDC. Multicolor flow analyze of pDC and cDC in human blood.
              Régis Josien, Nantes, France; Cecile Braudenau, Nantes, France
 14h30 - 17h30    Group 1 - Purification of human pDC and cDC. Multicolor flow analyze of pDC and cDC in human blood.
              Régis Josien, Nantes, France; Cecile Braudenau, Nantes, France
                          Group 2 - Generation of human DC from monocytes. 
              Séverine Remy, Nantes, France; Gaelle Tilly, Nantes, France
 
18h00 - 19h00     Immunohistology of human DC introduction.
 
 
Friday 2

09h00 - 10h00     Immunohistology of human DC.  
 
10h30 - 13h30     Group 1 - Generation of mouse DC from BM.
                          Marcelo Hill, Nantes, France; Séverine Remy, Nantes, France; Gaelle Tilly, Nantes, France
                          Group 2 - Purification of mouse pDC and cDC. Multicolor flow analyze of pDC and cDC in mouse spleen.
                          Laetitia Gautreau, Nantes, France; Virginie Tardif, Nantes, France 
14h30 -17h30      Group 1 - Purification of mouse pDC and cDC. Multicolor flow analyze of pDC and cDC in mouse spleen.
                          Laetitia Gautreau, Nantes, France; Virginie Tardif, Nantes, France
                          Group 2 - Generation of mouse DC from BM.
                          Marcelo Hill, Nantes, France; Séverine Remy, Nantes, France; Gaelle Tilly, Nantes, France


When
Arrival to Nantes June 30th evening; course will start in the morning of July 1st and finish late afternoon July 2nd; departure July 3rd 2010 (total of 2 days of course).

Where
Laboratories of the INSERM Unit 643 in Nantes University Hospital, Nantes, France. Nantes has an international airport and fast train line with Paris center city (2 h) and Charles de Gaulle airport (3 h).

 

Cost
The course is sponsored by ESOT (housing, lunches, registration), thus free of charge.
 
Basic Science Travel Grants
4 travel grants of up to 500 € are available – to apply please contact: ESOT Office – office@esot.org

Local scientific committee:
Ignacio ANEGON (ianegon@nantes.inserm.fr)
Maria Cristina CUTURI (ccuturi@nantes.inserm.fr)
Régis JOSIEN (rjosien@nantes.inserm.fr)
 
Local organization information:
Please, contact Valérie Chatellier by e-mail: Valerie.Chatellier@univ-nantes.fr  or by phone + 33 2400 87410. 
 
INSERM UMR 643-ITERT web site: http://www.ifr26.nantes.inserm.fr/ITERT/